Nov 27, 2019 · SRLV, a retrovirus that causes chronic and often subclinical infections, can influence the immune system of infected animals, as evidenced by changes in the expression of certain cytokines at the gene and protein level.
The CRISPR/Cas system can be implemented in lentivirus capable of infecting a broad variety of mammalian cells by co-expressing the a mammalian codon-optimized Cas9 nuclease along with a single guide RNA (sgRNA). A protocol for cloning into the lentiviral transfer
The Mu ltiple L entiviral E xpression (MuLE) is a modular and flexible system that can be used to construct complex lentiviruses for modification of mammalian cells with a single viral infection. This kit contains 96 plasmids and is shipped as bacterial glycerol stocks in a 96-well plate format. Lentivirus expression systems based on HIV-1 are becoming very popular for gene delivery into host cells, because they offer many advantages over both traditional retroviruses and adenoviruses. Lentiviruses can be used for either transient or stable expression, and they can infect both replicative and quiescent cells.
SMARTvector Inducible Lentiviral shRNA is the new market standard for inducible RNAi. Combining all of the advantages of the SMARTvector shRNA design innovations with the recently developed Tet-On 3G tetracycline-inducible expression system and the flexibility of SMARTchoice promoter and reporter options, this novel single-vector regulatable RNAi system permits the rapid development of stable ...
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